Technicality

  • Confocal microscopy is an optical imaging technique used to increase optical resolution and contrast of a micrograph by using point illumination and a spatial pinhole to eliminate out-of-focus light in specimens that are thicker than the focal plane.
  •  It enables the reconstruction of three-dimensional structures from the obtained images.

Used wavelengths
488, 532, 561, 632 nm stimulation
405 nm stimulation, for nucleus staining

 

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